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. Author manuscript; available in PMC: 2011 Apr 9.
Published in final edited form as: Biochem Biophys Res Commun. 2009 Nov 5;394(3):448–452. doi: 10.1016/j.bbrc.2009.10.169

Fig. 3. HPTLC analysis of acetone-extracted products from the [14C]-L-arginine to [14C]-L-citrulline conversion assay.

Fig. 3

Acetone-extracted samples that were passed through Dowex columns and the standards, L-arginine, L-citrulline and L-ornithine were separated by HPTLC in silica-60 gel plates and developed as described in Materials and Methods. Each sample contained all necessary substrates and cofactors for NOS activity. Samples were loaded in the following order: 1) nNOSr (30 nM); 2) MT (150 μg); 3) MT (150 μg) + Urease (50 mU); The migration of authentic standards of L-arginine, L-citrulline and L-ornithine, as detected by ninhydrin staining, is indicated on the right.