Fig.2.

LMP-1 decreases NO production and iNOS expression in LPS-treated RAW 264.7 macrophages/pre-osteoclasts. (A) A Nitrite assay was used to assess NO production. TAT-LMP-1 inhibited LPS-induced NO production in a concentration-dependent manner 24 h after LPS treatment. Data are presented as mean ± SEM from one representative experiment out of three independent experiments performed in triplicate. (B) Expression of iNOS protein was determined by Western blot using specific antibody. β-actin antibody was used to show equal protein loading. TAT-LMP-1 inhibited LPS-induced iNOS protein expression in a concentration-dependent manner. The image presented is from one representative experiment out of three independent experiments. Dentitometric quantification and statistical analysis include the results from three independent experiments. (C) The level of iNOS mRNA was detected by real-time RT-PCR. TAT-LMP-1 inhibited LPS-induced iNOS mRNA expression in a concentration-dependent manner. Data are presented as mean ± SEM of the fold change in mRNA levels from one representative experiment out of three independent experiments performed in triplicate. (*P < 0.05)