. Author manuscript; available in PMC: 2011 May 1.

Published in final edited form as: J Mol Cell Cardiol. 2010 Jan 15;48(5):954–963. doi: 10.1016/j.yjmcc.2010.01.002

Table 1.

Overview of cardiomyocytes force measurements

Donor
Incubation	F_max	pCa₅₀	ΔpCa₅₀	nH	Ktr
*Control*	*5 donor hearts; 13 myocytes*
Basal	26.8±3.8	5.43±0.03		2.7±0.2	0.67±0.07
After PKA	24.8±3.7	5.40±0.03	0.03±0.01	2.6±0.2^*	0.92±0.10^*

*cTn [0.5mg/ml]*	*5 donor hearts; 19 myocytes*
Basal	26.7±3.2	5.55±0.02		2.9±0.2	0.66±0.05
After PKA	23.1±2.5^*	5.46±0.02^*	0.10±0.01	2.8±0.2	0.69±0.04

*cTn [1.0mg/ml]*	*5 donor hearts; 14 myocytes*
Basal	21.1±3.2	5.50±0.02		2.6±0.2	0.64±0.03
After PKA	20.0±3.0	5.41±0.02^*	0.10±0.01	2.8±0.3	0.62±0.05
*Failing*
Incubation	F_max	pCa₅₀	ΔpCa₅₀	nH	Ktr

*Control*	*5 failing hearts; 15 myocytes*
Basal	31.5±3.8	5.60±0.01		3.6±0.3	0.59±0.04
After PKA	30.1±4.0	5.50±0.01^*	0.10±0.01	3.9±0.3	0.75±0.10

*cTn+P [0.5mg/ml]*	*5 failing hearts; 18 myocytes*
Basal	21.4±1.6	5.57±0.02		2.9±0.1	0.65±0.06
After PKA	18.4±1.5^*	5.44±0.02^*	0.13±0.01	2.9±0.1	0.66±0.06

*cTn+P [1.0mg/ml]*	5 failing hearts; 17 myocytes
Basal	20.9±2.3	5.60±0.02^*		3.3±0.2	0.71±0.10
After PKA	20.1±2.0	5.48±0.02	0.12±0.02	3.3±0.3	0.64±0.04

Control denotes results obtained from cardiomyocytes kept in exchange buffer without cTn complex added. Abbreviations: F_max, maximal force at saturating calcium concentration in kN/m²; pCa₅₀ and nH, midpoint and steepness of the force-pCa curves, respectively; Ktr, rate of tension redevelopment (s⁻¹) at saturating calcium concentration.

P<0.05, effect of PKA in paired t-test.