Skip to main content
. Author manuscript; available in PMC: 2011 Apr 15.
Published in final edited form as: Dev Biol. 2009 Oct 7;340(2):209–221. doi: 10.1016/j.ydbio.2009.09.042

Fig. 2.

Fig. 2

Chromosomal end-1,3 double mutants lack endoderm. (A) DIC appearance of end-1(ok558) end-3(ok1448) embryos. The embryo on the left is rescued by an extrachromosomal transgene array carrying end-1(+) and end-3(+) (referred to as an end-1,3(+) array). On the middle embryo, internal cavities are indicated by arrowheads. (B) Polarized light micrograph showing appearance of birefringent gut granules in the rescued embryo (left, outlined in red) but not in the mutant embryos (outlined in yellow). (C) end-1(ok558) end-3(zu247) embryos. The embryo on the left carries an end-1,3(+) array. (D) Gut granules present only in the rescued embryo (red outline). (E) Newly-hatched wild-type L1 (left) and arrested end-1(ok558) end-3(ok1448) double mutant. The gut in the wild-type has been shaded blue, and the pharynx and hindgut (rectum) have been shaded in red. The wild-type is approximately 240µm long. (F) Expression of elt-2::GFP (Fukushige et al., 1998) in a wild-type elongated embryo. DIC and fluorescence image have been digitally combined in this panel and panels G, H, J and K. (G,H) Absence of elt-2 expression in end-1(ok558) end-3(ok1448) and end-1(ok558) end-3(zu247) embryos. (I) Accumulation of gut granule-like material in part of the excretory cell (arrowheads) of an end-1(ok558) end-3(zu247) arrested embryo. (J) Expression of pha-4::GFP (Horner et al., 1998; Kalb et al., 1998) in a developing end-1,3 embryo carrying an end-1,3(+)array. The pharynx and hindgut (h.g.) components of pha-4 expression are indicated; the remaining expressing cells are from the developing intestine. (K) Absence of the intestinal component of pha-4 expression in an end-1(ok558) end-3(ok1448) embryo at a similar stage as panel J. (L) end-1(ok558) end-3(ok1448) embryos arrested with a one-fold appearance following heat shock overexpression of elt-2. Two embryos that carry an end-1,3(+) array are indicated. (M) Accumulation of large amounts of gut granules in rescued (red outline) and non-rescued (yellow outline) embryos. A C. elegans embryo is approximately 50µm long. In panels J and K, anterior is to the left and dorsal is up.