Table 3.
Production of tissues in laser-operated embryos
| genotype | blastomeres ablated or isolated |
partial embryos making tissues (# of cells) | |||
|---|---|---|---|---|---|
| coelomocytes (cup-4::GFP) |
pharynx muscle (myo-2::GFP)1 |
body muscle (unc-120::GFP) |
epidermis (nhr-25::YFP)1 |
||
| wild type | MS abl. | 0/3 (0.0 ± 0.0) | - | - | - |
| MS iso. | 3/3 (4.0 ± 0.0) | - | - | 0/1 | |
| E iso. | 0/6 (0.0 ± 0.0) | - | 0/4 (0.0 ± 0.0) | 0/6 | |
| C iso. | - | - | - | 5/5 | |
| skn-1(RNAi) | MS iso. | - | - | - | 3/3 |
| end-3(ok1448) | MS abl. | 0/5 (0.0 ± 0.0) | - | - | - |
| E abl. | 2/2 (4.0 ± 0.0) | - | - | - | |
| end-3(ok1448); pop-1(zu189)2 | ABa+MS abl. | - | 3/3 (weak) | - | - |
| ABa+E abl. | - | 2/2 | - | - | |
| ABa+MS+E abl. | - | 0/3 | - | - | |
| end-3(ok1448); pop-1(RNAi) | MS abl. | 4/4 (3.0 ± 0.6) | - | - | - |
| E abl. | 5/5 (3.0 ± 0.5) | - | - | - | |
| MS+E abl. | 1/4 (0.3 ± 0.2) | - | - | - | |
| end-1(ok558) end-3(ok1448) | MS abl. | 1/5 (0.2 ± 0.2) | - | - | - |
| E abl. | 5/5 (3.6 ± 0.2) | - | - | - | |
| MS+E abl. | 0/3 (0.0 ± 0.0) | - | - | - | |
| MS iso. | 5/5 (4.0 ± 0.0)3 | - | 3/3 (18.7 ± 2.0) | 0/53 | |
| E iso. | 0/4 (0.0 ± 0.0)3 | - | 3/3 (15.3 ± 0.7) | 10/103 | |
| pop-1(zu189)2 | MS abl. | - | 1/1 | - | - |
| ABa abl. | - | 0/3 | - | - | |
| pop-1(RNAi) | MS iso. | - | - | - | 0/3 |
| E iso. | - | - | - | 0/4 | |
| end-1(ok558) end-3(ok1448); | ABa+MS abl. | - | 5/5 | - | - |
| pop-1(zu189)2 | ABa+E abl. | - | 4/4 | - | - |
| ABa+MS+E | - | 0/3 | - | - | |
| end-1(ok558) end-3(ok1448); | MS abl. | 3/3 (6.7 ± 0.5) | - | - | - |
| pop-1(RNAi) | E abl. | 3/3 (4.7 ± 0.7) | - | - | - |
| MS+E abl. | 0/4 (0.0 ± 0.0) | - | - | - | |
| MS iso. | 4/4 (5.0 ± 0.4) | - | - | 0/8 | |
| E iso. | 6/6 (5.5 ± 0.3) | - | 6/6 (10.2 ± 1.2) | 9/11 | |
| ABx+MS abl. | - | 5/8 | - | - | |
| ABx+E abl. | - | 4/6 | - | - | |
1
Numbers of cells were not counted because individual cells could not be distinguished.
2
Embryos from homozygous pop-1(zu189) dpy-5(e61) mothers (with other mutations as indicated).
3
Numbers from experiments on a strain carrying both nhr-25::YFP and cup-4::GFP were included.
Abbreviations: abl, ablated; iso, isolated (all other blastomeres ablated); ABx, both daughters of AB were ablated.