Table 1.

Mixed-effect model of concentrations of antimicrobial proteins in the albumen of 176 blue tit eggs. laying order (LO), its quadratic effect (LO²) and clutch size (CS) were modeled as fixed effects, and nest of origin (Nest ID, n = 44) as a random effect

	Lysozyme			Avidin			Ovotransferrin
Parameter	df	F	P	df	F	P	df	F	P
LO	3,159	0.30	0.82	3,149	1.85	0.14	3,133	11.56	<0.01
LO2	3,159	0.79	0.50	3,149	0.99	0.39	3,133	12.76	<0.01
CS	1,159	1.08	0.30	1,151	2.01	0.15	1,133	0.10	0.75
LO x CS	3,159	0.24	0.86	3,151	1.94	0.12	3,133	0.60	0.61
Nest ID Wald Z:	2.05, p = 0.04			8.68, p <0.01			0.06, p = 0.97