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. 2010 Apr 5;189(1):69–81. doi: 10.1083/jcb.201001054

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© 2010 Albanèse et al.

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Figure 1. — Jjj1 and Zuo1–Ssz1 have overlapping biological functions. (A) Similar domain organization of Jjj1 and Zuo1. Jjj1 contains an N-terminal J domain, two zinc fingers (ZnF), and a C-terminal R/K-rich domain. Alignment with Zuo1 defines an additional homology domain (ZHD; Fig. S2). (B) Cells deleted for both Jjj1 and either Zuo1 (top) or Ssz1 (bottom) present a synthetic growth phenotype. An equal number of cells was spotted as a 10-fold dilution series on YPD plates and incubated at 30°C for 2 d. (C) Jjj1 overexpression does not suppress the antibiotics sensitivity of Δzuo1 cells. Δzuo1 cells were transformed with an empty p426 vector, the p426-ZUO1 and p426-JJJ1. The serial dilution was performed as in B.