Figure 9.

Inhibition of caveolar endocytosis prevents TNF-induced barrier dysfunction and fluid secretion. (A) In vivo perfusion assays of wild-type mice injected with TNF (gray bars) or vehicle (white bars). Inclusion of 50 µM dynasore or 2 mM MβCD within the perfusion solution prevented TNF-induced increases in BSA flux (n = 3). (B) The direction of water movement is reversed from net absorption to net secretion in wild-type mice injected with TNF (gray bars) relative to wild-type mice treated with vehicle (white bars). Dynasore prevented and MβCD reduced TNF-induced water secretion (n = 3). Error bars indicate mean ± SEM.