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. 2010 Apr 5;189(1):23–39. doi: 10.1083/jcb.200912163

Figure 2.

Figure 2.

Centrosomes detach from mitotic spindle poles in cnn1−/− and cnn2−/− cells. (A) wt and cnn1−/− cells are indistinguishable in prophase. Blue, DNA; green, pT288Aur-A; red, α-tubulin. (B) In wt cells, anti–centrin-3 antibody staining colocalizes with spindle pole regions marked by anti-TACC3 antibody (top). In mutant cells, centrosomes partially (yellow asterisks) or fully (blue asterisk) detach from spindle poles. Blue, DNA; red, TACC3; green, centrin-3. The graph shows quantification of the centrosome phenotypes seen in prometaphase/metaphase cells. (C) In anaphase cnn1−/− cells, pT288Aur-A staining is located near the cortex away from the spindle poles (arrow). Blue, DNA; green, α-tubulin; red, pT288Aur-A. The graph shows quantification of centrosome phenotypes in anaphase cells. (D) Centrosomal levels of tag-ΔCNN1 protein do not correlate with centrosome detachment in tag-cnn1lox cells. Arrow marks a detached centrosome. Blue, DNA; green, γ-tubulin; red, protein G. n = 4; 150 cells per experiment. Error bars represent SD. Bars, 5 µm.