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. 2010 Apr 15;24(8):783–798. doi: 10.1101/gad.1897310

Figure 3.

Figure 3.

BCL11A and SOX6 are coexpressed during erythroid development. (A) Human CD34+ cells were induced for erythroid differentiation in a serum-free two-phase liquid culture model. Representative cytospin images were shown for undifferentiated CD34+ cells, proerythroblasts (Pro-E), basophilic erythroblasts (Baso-E), polychromatic erythroblasts (Poly-E), and orthrochromatic erythroblasts (Ortho-E). (B) Globin mRNA levels were monitored by real-time RT–PCR in erythroid cells at various differentiation stages. All transcript levels were normalized against human GAPDH transcript levels. (C) The expression of human BCL11A and SOX6 proteins was measured by Western blot. GAPDH was analyzed as a loading control. (D) The CD71 and Ter119 expression pattern is shown for mouse fetal liver cells from E14.5 embryos. The cells were FACS-sorted into four populations (I, II, III, and IV) as described previously (Zhang et al. 2003). (E,F) Mouse Bcl11a and Sox6 mRNA levels were measured by real-time RT–PCR. Transcript levels were normalized against mouse Gapd transcript levels. All results are means ± SD of at least three independent experiments.