Nf1/nf1 mouse brain astrocytes show normal GFAP expression in thalamus and cerebellum. Sections through thalamus (A–D) or cerebellum (E–H) were stained with anti-GFAP antibody, and labeling visualized as described in Section 2. Sections from wild type mice are shown in A, B, E, and F. Sections from Nf1/nf1 mice are shown in C, D, G, and H. Low magnification photographs are shown in the left panel (Bar = 100 μM); higher magnification views are on the right (Bar =50 μm).