FIG. 6.

Scatchard analysis of anti-SSTR2A MAbs versus the D341 MED cell line. Iodinated anti-SSTR2A MAbs (¹²⁵I) and irrelevant IgG_2a (¹³¹I) in serially decreasing dilutions were reacted versus a fixed number of D341 MED cells for 4 h at 4°C; cells were pelleted and counted to determine percent of input specifically and nonspecifically bound. KA and β_max were determined by nonlinear regression analysis (Prism 4.0, Notepad Graphics); β_max was used to determine receptor number per cell. For presentation, data were transformed to yield the standard Scatchard plot (Prism 4.0) depicted here.