Table 2.
Results of Immunohistochemical Evaluation of Formalin-Fixed, Paraffin-Embedded Medulloblastoma Samples with MAb H5
| Case ID | Classificationa | Age (years) | Gender | Intensity/localization patternb |
|---|---|---|---|---|
| P93 | Diffuse | 5 | M | +2; cytoplasmic and membranous |
| P76 | Mixed diffuse and nodular with focal moderate anaplasia | 17 | M | Negative |
| M35 | Nodular | 14 | F | +3; cytoplasmic and membranous |
| P54 | Nodular | 14 | F | +2; cytoplasmic and membranous |
| S93 | Nodular | 11 | M | +1; cytoplasmic, focal |
| P10 | Nodular, focally anaplastic | 0.4 | F | +2; cytoplasmic |
| P44 | Nodular, early anaplasia | 5 | M | +1; cytoplasmic and membranous |
| P04 | Nodular, moderate anaplasia | 14 | M | +2; cytoplasmic and membranous |
| P57 | Diffuse, early anaplasia | 8 | M | +2; cytoplasmic, focal bv accumulation |
| T77 | Diffuse, severe anaplasia | 27 | M | +2; cytoplasmic, focal bv accumulation |
| P65 | Diffuse, moderate anaplasia | 16 | M | Negative |
| P27 | Diffuse, focal anaplasia | 6 | M | +2; cytoplasmic and membranous |
Nodular sheet-like profusion of tumor cells interrupted by nodules of small neurocytic-like cells producing a “pale island” appearance; diffuse sheets of cells not interrupted by nodular growth pattern; anaplasia, nuclear profiles characterized by elongation, duskiness of chromatin, increasing apoptotic figures, increasing mitotic figures, and cellular molding about other cells; moderate anaplasia, characterized by a “starry sky” appearance of apoptotic nuclei; severe anaplasia, exhibiting geographic necrosis.
Intensity pattern: negative, no detectable localization; +1, weakly intense; +2, moderately intense; +3, strongly intense. Localization pattern: cytoplasmic, presence of diffuse stain throughout cell cytoplasm; membranous, distinct decoration of membrane, cell-cell contact sites; focal blood vessel (bv) accumulation, apparent accumulation of positive cells around blood vessels. No nuclear staining was seen.