Figure 4.
Wild-type and Nf1-deficient Schwann cells express GEFs that specifically activate R-Ras and TC21/R-Ras2. cDNA was prepared from wild-type (+/+) and Nf1-null (−/−) MSCs. (a) RT–PCR was used to determine the expression of (b) GEFs that activate both Ras and R-Ras family members and (c) GEFs that specifically activate R-Ras family proteins. The + and − symbols denote the presence or absence of the message following a 40-cycle RT–PCR reaction. *Message was detectable only when using gene-specific cDNA. The RT symbol indicates presence or absence of the reverse transcriptase enzyme