Figure 4.

Structural modelling of human GlyT2. The wild-type (A) and the S510R mutant (B) model were generated based on homology with the crystal structure of LeuT, a bacterial Na⁺/Cl⁻-dependent neurotransmitter transporter homologue (PDB: 2A65). The models cover residues 191–754 of GlyT2 and show the position of the S510R mutation in TM7 along with the extensive re-arrangement of other transmembrane regions in the S510R that results in defective membrane trafficking of S510R and trapping of wild-type GlyT2 (see Rees et al., 2006). Models were visualized using the molecular graphics program Chimera (http://www.cgl.ucsf.edu/chimera/).