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. Author manuscript; available in PMC: 2010 Apr 14.
Published in final edited form as: Am J Physiol Renal Physiol. 2000 Nov;279(5):F927–F943. doi: 10.1152/ajprenal.2000.279.5.F927

Fig. 7.

Fig. 7

Protection by individual CAC intermediates and related metabolites delivered only during reoxygenation. At the end of 60 min hypoxia in the presence of 2 mM glycine at pH 6.9 tubules were resuspended in oxygenated medium at pH 7.4 with 2 mM glycine and 250 μM AMP. This suspension was divided in half so that one of the aliquots received no extra substrate (NES) and the other aliquot was supplemented with the indicated substrate or substrate combination (all additions 4 mM). ATP levels (panels A and C) and 590/530 nm JC-1 fluorescence ratios (panels B and D) measured after 60 min reoxygenation are means ± SE for N = 5–12, *significantly different from the corresponding paired NES group. Values for oxygenated control tubules incubated in parallel (not shown) were ATP, 20.0 ± 0.69 nmol/mg protein; 590/530 nm JC-1 fluorescence ratio, 4.38 ± 0.10. AcAc, acetoacetate; βOHB, β-hydroxybutyrate.