. Author manuscript; available in PMC: 2010 Apr 14.

Published in final edited form as: Biochemistry. 2008 May 23;47(24):6322–6328. doi: 10.1021/bi800075c

Table 1.

Sequence of Oligonucleotide Primers^a

H154N_f	GGCCACGGAATTATGTTCGACAATGCAACAGGTATTGTTGTGG
H154N_r	CCACAACAATACCTGTTGCATTGTCGAACATAATTCCGTGGCC
H189N_f^b	CGGGAAAAGAATCAGGCGATCGTAATCCCAAAGTACGCGAGGGTG
H189N_r^b	CACCCTCGCGTACTTTGGGATTACGATCGCCTGATTCTTTTCCCG
D139N_f	CAGTAGCTTTCGATGTAAATATTCACCCAGCGGCG
D139N_r	CGCCGCTGGGTGAATATTTACATCGAAAGCTACTG

Codons for mutation are in bold. Subscripts f and r represent forward and reverse primers, respectively.

These two primers were used to make the H154N/H189N double mutant, and the plasmid of H154 was the template.