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. 2009 Oct 28;118(4):479–484. doi: 10.1289/ehp.0901334

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PCB exposure decreases claudin-5 immunoreactivity in cerebral microvessels in mice 48 hr after treatment with individual PCB congeners (150 μmol/kg) or vehicle. Images were acquired using a 60× oil-immersion lens and confocal microscopy. Claudin-5 immunoreactivity is stained in red; staining with DAPI was performed to visualize the nuclei (stained in blue); phase contrast micrographs illustrate the isolated microvessels; and merged micrographs show localized claudin-5 immunoreactivity within the microvessels. Arrows illustrate areas of disrupted claudin-5 immunoreactivity; n = 3–4 per group.