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Spectral changes of nitroalkene reduction by PETN reductase under aqueous conditions. Inset: Time course of absorbance changes upon substrate reduction. The arrows indicate the direction of spectral change. (a) (Z)-2-(4′-chlorophenyl)-1-nitropropene [(Z)-4c]; (b) (E)-2-(4′-chlorophenyl)-1-nitropropene [(E)-4c]; (c) (Z)-1-(4′-chlorophenyl)-2-nitropropene [(Z)-5b]; (d) (E)-1-(4′-chlorophenyl)-2-nitropropene [(E)-5b].
