Table 1.
(a) Steady state kinetic constants and (b) rate constants for the oxidative half-reaction of PETN reductase with three nitro olefin substrates. In the case of (E)-2-3, kox/Kd was determined from the initial slope of the kobs vs. substrate concentration plot where the substrate concentration is well below Km
| (a) Steady State Compound | ||||||
|---|---|---|---|---|---|---|
| kcat [s-1] | PETN reductase Km [μM] |
kcat/Km [s-1M-1] | kcat [s-1] | OYE1[a] Km [μM] |
kcat/Km [s-1M-1] | |
| 1 | 9.5±1.0 | 971±146 | 9773 | 4.4±0.2 | 10±1 | 440,000 |
| (E)-2 | 20.9±0.8 | 341±42 | 61290 | 5.0±0.1 | 4.2±0.3 | 1,190,476 |
| (E)-3 | 21.4±1.0 | 413±39 | 51815 | 4.3±0.2 | 7.7±0.5 | 558,441 |
| (b) Oxidative Half Reaction Compound | ||||||
|---|---|---|---|---|---|---|
| kox [s-1] | PETN reductase Kd [mM] |
kox/Kd [s-1M-1] | kox [s-1] | OYE1[a] Kd [mM] |
kox/Kd [s-1M-1] | |
| 1 | 41.7±2.1 | 6.4±0.7 | 6502 | - | - | 610,000 |
| (E)-2 | ND | >3 mM | 4250 | 420±20 | 0.15±0.02 | 2,800,000 |
| (E)-3 | ND | >2 mM | 1272 | 170±30 | 0.17±0.03 | 1,000,000 |
[a]
Data from[27]
ND=not determined.