Table 2.

Reduction of various activated alkenes by PETNR using NADP⁺/G6PDH co-factor regeneration system.^[a]

Entry		Substrate	Conf.	Product	t [h]	Conv.[b] [%]	Yield[b] [%]	ee[c] [%]
1	1a		(R)-1b		24	>99	98	>99
2	2a		(R)-2b		24	>99	99	>99
3	3a		(R)-3b		24	>99	95	49
4	5a		(R)-5b		24	>99	93	98
5	(5R)-6a		(2R,5R)-6b		24	>99	82	95%[d,e]
6	(5S)-7a		(2R, 5S)-7b		24	>99	95	88%[d,e]
7	10a		(S)-10b		24	>99	85	66
8	11a		(S)-11b		24	>99	56	87
9	15a		(S)-15b		24	25	18	>99
10	16a		(S)-16b		24	14	14	>99
11	17a		(S)-17b		24	58	56	70
12	(E)-18a		(S)-18b		24	96	70	69
13	(Z)-18a		(S)-18b		24	>99	87	95
14	(E)-19a		(S)-19b		24	64	40	64

^[a]Conditions: Reactions contained potassium phosphate buffer (50 mM KH₂PO₄/K₂HPO₄ pH 7.0, 1.0 mL), alkene (5 mM), PETN reductase (2 μM), glucose 6-phosphate dehydrogenase (10 U), glucose 6-phosphate (15 mM) and NADP⁺ (10 μM). Reactions were agitated at 30 °C at 130 rpm for 24 h. Alkenes 1a, 3a and 18-19a were added as a DMF solution [2% (v/v) final DMF concentration].

^[b]by GC using DB-Wax column.

^[c]By GC or HPLC.