Table 5.
Enzyme concentration dependence for the reduction of ketoisophorone 3a after 2 h.[a]
| Entry | Enzyme concentra- tion |
Conv.[b] [%] |
Yield[b] [%] |
ee[c] [%] |
|---|---|---|---|---|
| 1 | 2 μM | >99 | 95 | 93 |
| 2 | 0.2 μM | >99 | 95 | 96 |
| 3 | 0.02 μM | 37 | 33 | 95 |
| 4 | 0.002 μM | 2 | 2 | >99 |
[a]
Conditions: alkene 3a (5 mM), PETN reductase, NADPH (6 mM) in phosphate buffer (50 mM, pH 7.0, 1 mL total volume), 30 °C at 130 rpm, 2 h. Alkene 3a was added as a DMF solution (2% final DMF solution).
[b]
By GC using a DB-Wax column.
[c]
By GC using Chirasil-DEX-CB.