Table 2. Mutational Studies of gp42.
Summary of effects of mutation on gp42 from three published studies. Symbols used: Plus sign (+) = functional in fusion assays; minus (−) = non-functional in fusion assays; plus/minus (+/−) = reduced function in fusion assays; Δ = deletion of residues following the symbol; ND = assay not done.
| Mutant | Functional Domain | Expressed at surface? | Fusion | Binding | Binding Partner | |
|---|---|---|---|---|---|---|
| Study 1a | LI12 | TM | Yes | + | + | HLA |
| LI27 | N-terminus | Yes | + | + | HLA | |
| LI82 | N-terminus | Yes | + | + | HLA | |
| LI93 | N-terminus | Yes | + | + | HLA | |
| W44A | N-terminus | Yes | + | + | HLA | |
| Q89A | N-terminus | Yes | + | + | HLA | |
| T104A | Class II | Yes | + | + | HLA | |
| R154A | Class II | Yes | + | + | HLA | |
| Y185F | Yes | + | + | HLA | ||
| LI118 | Structural | Yes | − | − | HLA | |
| LI122 | Structural | Yes | − | − | HLA | |
| LI134 | Structural | Yes | +/− | +/− | HLA | |
| LI179 | Structural | Yes | − | − | HLA | |
| LI195 | Yes | − | − | HLA | ||
| LI216 | Structural | Yes | − | − | HLA | |
| W125G | Structural | Yes | − | − | HLA | |
| LI104 | Class II | Yes | − | − | HLA | |
| LI112 | Class II | Yes | − | − | HLA | |
| LI148 | Class II | Yes | − | − | HLA | |
| LI149 | Class II | Yes | − | − | HLA | |
| Y107A | Class II | Yes | − | − | HLA | |
| E160A | Class II | Yes | − | − | HLA | |
| R220A | Class II | Yes | +/− | − | HLA | |
| LI193 | Yes | − | + | HLA | ||
| LI206 | Yes | − | + | HLA | ||
| LI210 | Yes | +/− | + | HLA | ||
| F210A | Yes | − | + | HLA | ||
| Study 2b | R30A | N-terminus | Yes | + | + | gH/gL |
| R32A | N-terminus | Yes | + | + | gH/gL | |
| R36A | N-terminus | Yes | + | + | gH/gL | |
| W44A | N-terminus | Yes | + | + | gH/gL | |
| K47A | N-terminus | Yes | + | + | gH/gL | |
| K47A/P48S | N-terminus | Yes | + | + | gH/gL | |
| E51A | N-terminus | Yes | + | + | gH/gL | |
| Δ32–36 | N-terminus | Yes | + | + | gH/gL | |
| Δ57–61 | N-terminus | Yes | + | − | gH/gL | |
| Δ62–66 | N-terminus | Yes | + | + | gH/gL | |
| Δ37–41 | N-terminus, cleavage | Yes | − | + | gH/gL | |
| Δ42–46 | N-terminus | Yes | − | + | gH/gL | |
| Δ82–86 | N-terminus | Yes | − | + | gH/gL | |
| Δ87–91 | N-terminus | Yes | − | + | gH/gL | |
| Δ92–96 | N-terminus | Yes | − | + | gH/gL | |
| Δ47–51 | N-terminus | Yes | − | +/− | gH/gL | |
| Δ52–56 | N-terminus | Yes | − | − | gH/gL | |
| Δ67–71 | N-terminus | Yes | +/− | − | gH/gL | |
| Δ72–76 | N-terminus | Yes | − | − | gh/gL | |
| Δ77–81 | N-terminus | Yes | − | +/− | gH/gL | |
| Δ45–89 | N-terminus | Yes | − | − | gH/gL | |
| Δ37–41/Δ67–71 | N-terminus | Yes | − | − | gH/gL | |
| Δ42–46/Δ72–76 | N-terminus | Yes | − | − | gH/gL | |
| Δ77–81/Δ47–51 | N-terminus | Yes | − | − | gH/gL | |
| Study 3c | ΔN36 | N-terminus | No; +gH/gL Yes | + | +, + | HLA, gH/gL |
| ΔN41 | N-terminus | No; +gH/gL Yes | + | +, + | HLA, gH/gL | |
| ΔN46 | N-terminus | No; +gH/gL Yes | + | +, + | HLA, gH/gL | |
| ΔN51 | N-terminus | No; +gH/gL No | − | +, − | HLA, gH/gL | |
| VAAAA37–41DDDDK | N-terminus, cleavage | ND | +/− | ND | ||
a
A.L. Silva, J. Omerovic, T.S. Jardetzky, R. Longnecker, J Virol 78, 5946–5956, (2004);
b
A.N. Kirschner, A.S. Lowrey, R. Longnecker, T.S. Jardetzky, J Virol 81, 9216–9229, (2007);
c
J. Sorem, T.S. Jardetzky, R. Longnecker, J Virol 83, 6664–6672, (2009)