Induction of KLF4 gene expression by DIM-C-pPhC6H5 and the 5,5’-dihydroxy and 5,5'-dimethyl analogs in SW480 and HT-29 cells. Induction of KLF-4 (A) and inhibition by T007 (B). Cells were treated with different concentrations of DIM-C-pPhC6H5 and the 5,5’-dihydroxy and 5,5'-dimethyl analogs or T007 alone or in combination for 24 hr, and KLF4 mRNA levels were determined by real-time polymerase chain reaction as described in the Materials and Methods. Each experiment was replicated ( > 3X). T007 did not inhibit KLF4 mRNA induction by DIM-C-pPhC6H5 and the 5,5’-dihydroxy and 5,5'-dimethyl analogs.