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. 2010 Mar 31;354(1-2):68–79. doi: 10.1016/j.jim.2010.02.002

Fig. 1.

Fig. 1

Comparison of polyclonal expansion methods for expanding T cells from blood. PBMC were expanded using seven different combinations of expansion protocols and cytokine cocktail: anti-CD3/IL-2 (dark blue), anti-CD3/anti-CD28/IL-2 (medium blue), anti-CD3/IL-2/IL-7/IL-15 (light blue), Dynal anti-CD3/CD28 beads (3:1)/IL-2 (yellow), Dynal anti-CD3/CD28 beads (1:5)/IL-2 (light orange), Dynal anti-CD3/CD28 beads (1:1)/IL-2 (dark orange), and Dynal anti-CD3/CD28 beads (1:1)/IL-2/IL-7/IL-15 (red). Bars represent the median of 5 donors' cell recovery after 3, 5 and 7 days. Error bars represent the interquartile range for measurements taken from five donors. The figures on top of the bars represent median fold expansion after culturing. Wilcoxon rank test was used for comparison of day 0 and day 7 yields.