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. 2005 Apr 30;1(1):81–91. doi: 10.3988/jcn.2005.1.1.81

Figure 3.

Figure 3

Effect of β-carbolines and R-(-)-deprenyl on SNAP-induced nuclear damage. PC12 cells were treated with 500 µM SNAP in the presence of 25 µM β-carbolines or R-(-)-deprenyl for 24 hours. In the experiment (A) cells were observed by fluorescence microscopy after nuclei staining with Hoechst 33258. a: control cells, b: cells treated with SNAP alone, c: cells treated with SNAP and harmine, (d) cells treated with harmine alone. All the subparts are representative of four different experiments. In the experiment (B) the 3'-ends of DNA fragments were detected as described in Materials and Methods. Data are expressed as absorbance and represent the means±SEM of 6 replicate values in two separate experiments. †P<0.05, compared to control. *P<0.05, compared to SNAP alone.