Fig. 1.

Phospho-tyrosine proteins increase at MII and localize primarily to the oocyte cortex and within large cytoplasmic patches. Complete serial z-sections of oocytes labeled with pTyr and imaged in sets were examined to identify patterns of pTyr proteins associated with cellular structures at each time point of maturation (H0=GV, H2=GVBD, H4=pre-MI, H8=MI, H16=MII). pTyr labeled oocytes are shown here in the plane where chromatin was found. Two to three sections have been compressed together to enable visualization of entire structures such as both poles of the spindles. Image intensity has been increased in this figure to improve visualization of labeled structures. (white=pTyr, red=DNA)