Fig. 6.

MPM2 proteins increase during maturation and concentrate in the cytoplasm beneath the oolema. Oocytes labeled for MPM2 proteins were compared by linescans similar to pTyr images (see Fig. 3). Linescans across the median (a, c, e and f) demonstrate the steady increase of MPM2 proteins in both the central cytoplasm (*****) and the cortex (*) as oocytes mature from GV to MII. Fluorescence intensity was too low to measure at GV stage (a–b). The cortical (b, d, f and h) scans further demonstrate the increase in MPM2 proteins especially at MI (f) and MII (h). (* oolema/cortex; ***** central cytoplasm; A–B=GV, C–D=pre-MI, E–F=MI, G–H=MII)