Fig. 8.

Fyn (-/-) oocytes exhibit reduced levels of pTyr proteins in the cortex while MPM2 proteins localize normally the spindle and spindle poles. Fyn (-/-) and wildtype oocytes were matured in IVMh for 17 h, fixed and labeled for pTyr and MPM2 epitopes. The images of MPM2 labeled spindles (a–b) are compressions of 2–3 sections to show the entire spindle region however, pTyr images (c–d) are single sections from the central plane of each oocyte. There was no difference in MPM2 labeling on MII spindles or spindle poles of wildtype (a) or Fyn (-/-) (b) oocytes. However, Fyn (-/-) oocytes often failed to develop intense pTyr at the egg cortex or the spindle poles. As reported previously, Fyn (-/-) oocytes often produce MII oocytes with disorganized chromosomes (d) [14]. (A–B; white=MPM2, red=DNA: C–D; white=pTyr, red=tubulin, white on red=DNA)