Fig. 9.

MPM2 proteins aggregate near to chromatin within the GV and associate with spindle microtubules and spindle poles. Oocytes from the MPM2 fluorescence intensity comparisons were examined for localization of MPM2 proteins to intracellular structures. Images in this figure were extracted from these data sets including compressions of 2–3 sections to enable visualization of the entire structure. Dense patches of MPM2 proteins were seen to associate with chromatin within the GV stage oocytes. The specific pattern of this labeling was diverse. As chromosomes condensed at GVBD and began to organize at pre-MI, MPM2 patches were seen to remain near to the chromosomes. At MI and MII, MPM2 localized to the spindle microtubules and the spindle poles. (green=MPM2, red=DNA)