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. Author manuscript; available in PMC: 2010 Apr 15.
Published in final edited form as: Electrophoresis. 2009 Oct;30(20):3598–3606. doi: 10.1002/elps.200900259

Figure 2.

Figure 2

Azide-GG-OH is a substrate for protein geranylgeranylation in vivo. (A) Labeling and detection of geranylgeranylated proteins in Jurkat cells. Jurkat cells were metabolically labeled with DMSO, N3-GG-OH (G) or N3-F-OH in the presence of lovastatin (FL, where F stands for N3-F-OH) for 48 h. (B) and (C) Labeling and detection of geranylgeranylated proteins in other cell lines. Metabolic labeling experiments of COS-7 (B) and MCF-7 (C) were performed using N3-GG-OH (G), N3-F-OH (F) in the presence or absence of lovastatin (L) as indicated.