Table 1.
Identification of TAMRA-labeled, geranylgeranylated proteins in MCF-7 cellsa)
| Protein name | CAAX, CC, or CXC motif |
Protein molecular weight (AMU) |
% Coverage of protein in excised gel spots |
|---|---|---|---|
| RAB7, member of RAS oncogene family | SCSC | 23 472.00 | 28 |
| RAB11B | CQNL | 24 417.90 | 25 |
| RAB14, member of RAS oncogene family | GCGC | 23 909.60 | 25 |
| RAB1A, member of RAS oncogene family | GGCC | 22 660.40 | 25 |
| RAB3D, member of RAS oncogene family | SCSC | 24 249.50 | 26 |
| Ras-related small GTP-binding protein RAB5 | CCSN | 23 549.90 | 17 |
| RAB6A, member of RAS oncogene family | GCSC | 23 531.40 | 24 |
| RAB2, member of RAS oncogene family | GGCC | 23 528.20 | 25 |
| RAP2C, member of RAS oncogene family | CVVQ | 20 754.40 | 40 |
| RAB18, member of RAS oncogene family | CSVL | 22 959.50 | 26 |
a)
Excised spots (26 in total) from Sypro Ruby-stained gels were digested with trypsin, and the resulting tryptic peptides were subjected to MS. TAMRA-labeled, geranylgeranylated proteins were identified by peptide sequencing with the search programs MASCOT and X! Tandem.