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Gap-PCR for α-thalassemia deletions. The amplification products are run on 2% agarose gel. The normal (αα/αα) control shows two bands at 446 and 298 bp, while both parents show three bands (561, 446, and 298 bp) and are thus heterozygous for the Mediterranean deletion (αα/--MED). The infant shows only a single band at 561 bp and thus it is homozygous for the latter deletion (--MED/--MED).
