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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Scand J Immunol. 2010 Apr;71(4):232–239. doi: 10.1111/j.1365-3083.2010.02369.x

Figure 3.

Figure 3

A. Effect of single tyrosine mutations on FcγRIIA mediated serotonin secretion. Serotonin release was determined as described in Materials and Methods. Bars represent the percent of total available serotonin released and are the average of at least three separate experiments, each carried out in triplicate. IV.3 + GAM indicates crosslinking with both primary and secondary antibodies; MED indicates mock-stimulation with media alone; GAM indicates incubation with 2° antibody; IV.3 indicates incubation with primary antibody alone. (* p<0.05 compared to wildtype FcγRIIA-mediated release)

B. Effect of double tyrosine mutations on FcγRIIA mediated serotonin secretion. Serotonin release was determined as described in Materials and Methods. Bars represent the percent of total available serotonin released and are the average of at least three separate experiments, each carried out in triplicate. IV.3 + GAM indicates crosslinking with both primary and secondary antibodies; MED indicates mock-stimulation with media alone; GAM indicates incubation with 2° antibody; IV.3 indicates incubation with primary antibody alone. (* p<0.05 compared to wildtype FcγRIIA-mediated release)