Table 1. Peptides Detected Directly from Supraoptic Magnocellular and Hippocampal Neurons in Coculturea.
prohormone | obsd (m/z) | theor. (m/z) | error (Da) | putative magnocellular neuron | putative hippocampal neuron | presence in supraoptic nucleus (MSMS)b |
---|---|---|---|---|---|---|
neurokinin A [98−107] | 1132.87 | 1132.55 | −0.32 | x | ||
POMC[103−120]/J peptide | 1882.93 | 1882.83 | −0.10 | x | x | x |
neurokinin B[95−116] | 2345.58 | 2344.51 | −1.48 | x | ||
provasopressin[24−32] | 1084.49 | 1084.44 | −0.05 | x | x | |
provasopressin[24−33] | 1142.49 | 1142.46 | −0.03 | x | neuropred-rat model | |
provasopressin[158−168] | 1250.49 | 1250.37 | −0.86 | x | x | |
provasopressin[36−54] | 1920.36 | 1920.20 | −0.16 | x | neuropred-rat model | |
secretogranin2[571−583] | 1354.49 | 1354.67 | 0.18 | x | x | |
thymosin beta-10 | 4932.10 | 4932.51 | 0.41 | x | x | x |
thymosin beta-4 | 4959.30 | 4960.48 | 1.18 | x | x | x |
unknown | 863.74 | x | x | |||
unknown | 1021.50 | x | ||||
unknown | 1044.75 | x | x | |||
unknown | 1521.62 | x | ||||
unknown | 1586.61 | x | x | |||
unknown | 1799.60 | x | ||||
unknown | 1870.50 | x | ||||
unknown | 1997.85 | x | ||||
unknown | 2256.34 | x | ||||
unknown | 2481.59 | x | ||||
unknown | 2808.30 | x | x | |||
unknown | 2923.80 | x | ||||
unknown | 2985.70 | x | x | |||
unknown | 3004.60 | x | ||||
unknown | 3056.38 | x | x | |||
unknown | 3516.44 | x | x | |||
unknown | 4174.70 | x |
Summary of direct MALDI-TOF MS results for magnocellular neurons and hippocampal neurons in serum-free coculture after a single-step application of DHB matrix.
Reference (1).