Fig. 1. Laser-assisted microdissection of mesenchyme in the E13.5 mouse bladder. (HE w/o LCM).
Morphology of HE staining on serial section for laser capture microdissection. (HS w/ LCM) Morphology of HS staining after laser capture microdissection. Mesenchymal locations were chosen either immediately adjacent to the urothelium in the prospective submucosa or next to the serosal layer in the peripheral bladder mesenchyme, an area of future smooth muscle differentiation in E12.5 and E13.5 bladders. a, Serosal zone. b, Submucosal zone. E, Epithelium. Scale bar, 50μm.
