Morphology of HE staining on serial section for laser capture microdissection. (HS w/ LCM) Morphology of HS staining after laser capture microdissection. Bladder mesenchymes were captured in the submucosa and in the area of peripheral smooth muscle in E15 and E16. a1, Smooth muscle layer. a2, Intermediate zone. b, Submucosal zone. E, Epithelium. Scale bar, 50μm.