Fig. 3. Real time RT-PCR analysis on mRNA expressions of SMAA and SM-MHC in laser captured mesenchymal components of each gestational stage.
Expression of SMAA mRNA was detectable in the peripheral cellular population at E13.5 and increased to significantly high levels in smooth muscle cells at E15 and E16. SM-MHC was detectable temporal slightly later than SMAA and increasing in expression at E15 and E16 (Mann-Whitney test, p<0.001). a, Serosal zone. a1, Smooth muscle layer. a2, Intermediate zone. b, Submucosal zone.
