Fig. 5. Real time RT-PCR analysis on mRNA expression of TGF-β1 in laser captured mesenchymal components of each gestational stage.
TGF-β1 expression was minimal in the E12.5 bladder mesenchyme. TGF-β1 increased to the highest level in the smooth muscle differentiation layer adjacent to the serosa at E13.5 and then decreased in all mesenchymal compartments in the E15 and E16 (Mann-Whitney test, p<0.001). a, Serosal zone. a1, Smooth muscle layer. a2, Intermediate zone. b, Submucosal zone.
