Fig. 6. Real time RT-PCR analysis on mRNA expression of SRF in laser captured mesenchymal components of each gestational stage and mouse bladder developmental illustrations.
(A) SRF was significantly up regulated in smooth muscle cells at E15 compared to other mesenchymal components at E12.5, E13.5, and E16 (Mann-Whitney test, p<0.001). (B) Mouse bladder developmental illustrations with HE staining show locations of a1, a2 and b nomenclatures. a, Serosal zone. a1, Smooth muscle layer. a2, Intermediate zone. b, Submucosal zone. E, Bladder epithelium. Scale bar, 100μm.
