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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Cancer Res. 2009 Sep 29;69(19):7681–7688. doi: 10.1158/0008-5472.CAN-09-2133

Fig 1. ASK1 increases the Daxx expression by preventing proteasome-dependent degradation.

Fig 1

(a) TNFα treatment increases the expression level of Daxx. HeLa cells were treated with TNFα at the indicated concentrations for 18 h. The cell extracts were subjected to western blotting for Daxx and actin. (b, c) ASK1 stabilizes Daxx. 293 cells were transfected with expression plasmids for EGFP-Daxx, either full length (b) or a deletion mutant (70–216) (c), and EGFP together with control vector (3.1), ASK1-HA or kinase inactive mutant K709M (KM)-HA, and then cultivated for 2 days. Twelve hours after the treatment with or without proteasome inhibitors (PIs), cell extracts were prepared and subjected to western blotting with anti-EGFP antibody. The relative EGFP-Daxx protein levels are shown at the bottom as a ratio over the EGFP protein levels. NT: no treatment. Note that the expression level of EGFP serves as an internal reference of ectopic EGFP fusion protein expression.