Fig. 6.
Gα13Q226L induces the translocation of HDAC5 from nucleus to cytoplasm. HUVECs grown on 24-mm coverslips were transfected with 100 ng EE-tagged Gα13Q226L or Gα12Q229L and HA-tagged HDAC5. The expressions of Gα13Q226L and Gα12G229L were examined with EE antibody and anti-mouse secondary antibody conjugated to immunofluorescent dye Alexa 594 (red fluorescence). HDAC5 was detected with FITC-conjugated HA antibody. The nuclei were identified with DAPI. Two additional experiments showed similar results