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. Author manuscript; available in PMC: 2011 Feb 23.
Published in final edited form as: Biochemistry. 2010 Feb 23;49(7):1404–1417. doi: 10.1021/bi902132u

FIGURE 1.

FIGURE 1

HPLC purification of peptides from RTPR inactivated with [1′-3H]-F2CTP, treated with NaBH4 at 2 min, alkylated with iodoacetamide and digested with trypsin. A214nm (—), cpm (- - -). (A) Initial purification, linear gradient (……) 0.1% TFA, 0–45% CH3CN over 90 min. Region I, 50–53 min, 18.1% of radioactivity. Region II, 54–59 min, 19.7% of radioactivity. Region III, 60–63 min, 13.9% of radioactivity. Region IV, 63–67 min, 26% of radioactivity. (B) repurification of region I, linear gradient (……) 10 mM NH4OAc, pH 6.8, 0–35% CH3CN over 90 min. 80% recovery of radiolabel.