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. Author manuscript; available in PMC: 2011 Feb 23.
Published in final edited form as: Biochemistry. 2010 Feb 23;49(7):1404–1417. doi: 10.1021/bi902132u

FIGURE 4.

FIGURE 4

(A) MS/MS of peak at 2063 Da, containing a label derived from F2CTP. The y series is marked, and corresponds to the C-terminal peptide of RTPR (DLELVDQTDCEGGACPIK). The absolute MWs detected are shifted +43 Da relative to the acetamide labeled C-terminal peptide (Figure S1), suggesting alkylation at cysteine by a group 43Da larger than acetamide. The modification is present to some extent on each cysteine, as indicated by the appearance of the y series of the normal peptide after C731 is cleaved (indicated at 831, 702, 645, 588 and 517 Da).