FIG. 3.

Detection of cMPRO–Fc protein expression. (a) Western blot analysis demonstrates the expected band from the treated muscles of dog Ramone. (b) Immunofluorescence staining against dog MPRO indicates that cMPRO–Fc is expressed in treated muscle. Scale bar: 100 μm. (c) ELISA analysis of injected dog (Ramone) serum. cMPRO–Fc was detected from serum 1 week after vector delivery (900 pg/ml). Four weeks after vector delivery, the expression serum level of cMPRO–Fc in Ramone was reduced to about 600 pg/ml.