FIG. 3.

SREBP1 silencing in liver of db/db mice. Mice received 1.0 × 10¹¹ VP of gAd.shSREBP1 or gAd.shSCR, and were killed 3 weeks later. (A) Western blot analysis was used to determine the level of knockdown. Left: db/db mice have higher levels of SREBP-1 compared with lean (C57BKS) mice. Right: db/db mice administered gAd.shSREBP1 vector display a 90% reduction in SREBP-1 levels compared with db/db animals that received the gAd.shSCR vector. (B) Densitometric analysis of Western blot. (C) Aspartate aminotransferase (AST) levels in the serum of mice 3 weeks after gene transfer. No significant difference was observed between the two groups. SREBP-1, sterol regulatory element-binding protein-1; Cyp, cyclophilin.