FIG. 7.
In vivo antibody depletion experiment. Groups of C57BL/6 mice (five per group) were inoculated with MOSEC/luc (2 × 105 per mouse) and treated with PBS or CpG-ODN plus LL-37 on days 4, 8, and 12 as depicted in Fig. 3. Mice were either depleted of peritoneal macrophages, using clodronate liposomes, or of NK cells, using anti-mouse NK1.1 monoclonal antibody (PK136), 1 day before and 3 days after the first treatment and thereafter once per week until the end of follow-up as described in Materials and Methods. Undepleted mice were used as a negative control. Mice were imaged with the IVIS imaging system series 200 every 7 days. Bioluminescence signals were acquired for 1 min. Shown is a line graph depicting the quantification of luminescence activity in the tumors of tumor-challenged mice treated with PBS or with CpG-ODN plus LL-37 and depleted of peritoneal macrophages or NK cells (means ± SD).