FIGURE 3 .

Phosphorylation of IRE1α (A), ratio of spliced (s) to unspliced (u) XBP1 mRNA (B), and XBP1 protein in nuclear extracts (C) in the liver of FD, VEH, or RAP rats. Meal-fed rats were killed 1 h (VEH1, RAP1) or 7 h (VEH7, RAP7) after the 3-h meal-feeding period. Representative western blots indicate the proteins detected with antibodies against p-IRE1α, XBP1, actin, or Lamin A/C. Representative gel illustrates unspliced XBP1 and XBP1s mRNA. Values are the mean ± SE, n = 10. Means without a common letter differ, P < 0.05. *Different from FD, P < 0.05.