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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: Anticancer Drugs. 2009 Jul;20(6):444–449. doi: 10.1097/CAD.0b013e32832afc04

Fig. 2.

Fig. 2

Fig. 2

Fig. 2

Fig. 2

FLLL11 and FLLL12 inhibit STAT3 and/or AKT phosphorylation and induce apoptosis in (a) PANC-1, (b) BXPC-3, and (c) HPAC pancreatic cancer cell lines. However, FLLL11, FLLL12 do not increase cleaved caspase-3 in (d) human pancreatic duct epithelial cells. Cells were treated with 5–10λμmol/l of FLLL11 and FLLL12 as well as 10 or 20λμmol/l of curcumin for 24–48λh. Fifty micrograms of total protein from cell lysates were subjected to SDS polyacrylamide gel electrophoresis and transferred onto polyvinylidene fluoride membrane. Membranes were blotted with phospho-specific STAT3 (Tyrosine 705), phospho-independent STAT3, phospho-specific AKT antibody (Serine 473), cleaved PARP antibody, cleaved caspase-3 antibody, and GAPDH antibody. DMSO, dimethylsulfoxide.