Table 1. Gene-specific primer pairs used to characterise the different AhMTP1 paralogues in real-time quantitative RT–PCR analyses.

Gene	Primer name	Primer sequence 5′--3′	Annealing temperature (°C)	Average PCR efficiencies
				gDNA	cDNA
Actin	ActinF	GGTAACATTGTGCTCAGTGGTGG	65.9	1.87	1.90
	ActinR	AACGACCTTAATCTTCATGCTGC
AhMTP1-A1 &A2	ARTF	AGTGGTGAACATCATAATGGCTGTTC	67.2	1.96	1.93
	ACRTR	TCGATTTGTCCAACAGTTGCTCAG
AhMTP1-B	BRTF	TGGACGTGAAGTTATGGAGAC	64.3	2.06	2.04
	BRTR	CTTCACATTTGGGCTATCACAG
AhMTP1-C	CRTF	GGTGAACATCATAATGGCTGTTATGCTG	67.8	1.98	NP a
	ACRTR	TCGATTTGTCCAACAGTTGCTCAG
AhMTP1-D	DRTF	GTACACCCAGAGAGATTGACGCCG	71.3	1.91	NP a
	DRTR	GACTAATGTTGTACTCCCTGCGGATG

a NP: Not possible to be done (see Materials and Methods).